Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Opa1 and Mfn2.
Boopathy S, Luce BE, Lugo CM, Hakim P, McDonald J, Kim HL, Ponce J, Ueberheide BM, Chao LH
J Biol Chem. 2024 Aug 31:107740. doi: 10.1016/j.jbc.2024.107740.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227599 | pPICZ A-SLC25A46-His (SB250) | Inducible expression of His-tagged SLC25A46 in Pichia pastoris |
| 227600 | pPICZ A-Strep-Opa1 (SB251) | Inducible expression of Strep-tagged Opa1 in Pichia pastoris |
| 227601 | pPICZ A-Strep-Opa1(MGD) (SB252) | Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris |
| 227602 | pPICZ A-MFN2-Strep (SB253) | Inducible expression of Strep-tagged MFN2 in Pichia pastoris |
| 227603 | pPICZ A-SLC-His+Strep-Opa1 (SB254) | Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris |
| 227604 | pPICZ A-SLC-His+MFN2-Strep (SB255) | Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris |
| 227605 | pPICZ A-SLC25A46[I349D]-His (SB256) | Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris |
| 227606 | pPICZ A-SLC25A46[R247A,R347A]-His (SB257) | Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris |
| 227607 | pPICZ A-SLC25A46[∆2-83]-His (SB258) | Inducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastoris |
| 227608 | pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259) | Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris |
| 227609 | pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260) | Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris |
| 227610 | pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261) | Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris |
| 227611 | pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262) | Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris |
| 227612 | pET-28-Strep-OPA1(253-960) (SB263) | Inducible expression of Strep-tagged Opa1(253-960) in E.coli |
| 227613 | pET-28-Strep-OPA1(MGD) (SB264) | Inducible expression of Strep-tagged Opa1(MGD) in E.coli |
| 227614 | pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9) | Overexpression of SLC25A46 in mammalian cell lines |
| 227616 | pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11) | Overexpression of SLC25A46 with R257Q mutation in mammalian cell lines |
| 227617 | pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12) | Overexpression of SLC25A46 with R340C mutation in mammalian cell lines |
| 227618 | pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13) | Overexpression of SLC25A46 with I349D mutation in mammalian cell lines |
| 227619 | pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14) | Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines |
| 227620 | pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36) | Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines |