In situ architecture of Opa1-dependent mitochondrial cristae remodeling.
Fry MY, Navarro PP, Hakim P, Ananda VY, Qin X, Landoni JC, Rath S, Inde Z, Lugo CM, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH
EMBO J. 2024 Feb;43(3):391-413. doi: 10.1038/s44318-024-00027-2. Epub 2024 Jan 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226986 | pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7) |
| 226987 | pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6) |
| 226988 | pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9) |
| 226989 | pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b |
| 226990 | pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b |
| 226991 | pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b |
| 226992 | pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93) | pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b |