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A Genetically Encoded Photocaged Cysteine for Facile Site-Specific Introduction of Conjugation-Ready Thiol Residues in Antibodies.

Osgood AO, Roy SJS, Koo D, Gu R, Chatterjee A
Bioconjug Chem. 2024 Apr 17;35(4):457-464. doi: 10.1021/acs.bioconjchem.3c00513. Epub 2024 Mar 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226836pcDNA3.1-AntiHer2-HC121-TAGContains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation
226838pcDNA3.1-AntiHer2-HC197-TAGContains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation
226839pcDNA3.1-AntiHer2-LC122-TAGContains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Antibodies from Article