Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner.
Kamoku C, Nielsen DR
Biotechnol Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226793 | pCMK83 | Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B |
| 226803 | pCMK103 | Integrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology arm |
| 226804 | pCMK104 | Integrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology arm |
| 226805 | pCMK105 | Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm |
| 226818 | pCMK195 | Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms |
| 226851 | DHIP1 | Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria |