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Efficient gene editing of a model fern species through gametophyte-based transformation.

Jiang W, Deng F, Babla M, Chen C, Yang D, Tong T, Qin Y, Chen G, Marchant B, Soltis P, Soltis DE, Zeng F, Chen ZH
Plant Physiol. 2024 Sep 12:kiae473. doi: 10.1093/plphys/kiae473. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226706pRGEB32-CrU6-2-35SFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.
226707pRGEB32-CrU6-2-UBIFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.
226708pRGEB32-CrActin-35SFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.
226709pRGEB32-CrActin-UBIFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Antibodies from Article