A massively parallel in vivo assay of TdT mutants yields variants with altered nucleotide insertion biases.
Carlson CK, Loveless TB, Milisavljevic M, Kelly PI, Mills JH, Tyo KEJ, Liu CC
bioRxiv [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 10.1101/2024.06.11.598561.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226622 | pCKC017 | pLenti wt hTdT template for libraries 1-2 |
| 226623 | pCKC014 | pLenti wt hTdT template for library 3 |
| 226625 | pCKC764 | piggyBAC pCAG-hCas9-T2A-Unbiased3 |
| 226626 | pCKC775 | piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2 |
| 226627 | pCKC776 | piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1 |
| 226628 | pTBL435 | pcDNA-dTdT |