SNAP-tag2 for faster and brighter protein labeling.
Kühn S, Nasufovic V, Wilhelm J, Kompa J, de Lange EMF, Lin YH, Egoldt C, Fischer J, Lennoi A, Tarnawski M, Reinstein J, Vlijm R, Hiblot J, Johnsson K
Nat Chem Biol. 2025 Jul 3. doi: 10.1038/s41589-025-01942-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226512 | pET51b(+)_SNAP-tag2 | Bacterial cell expression of SNAP-tag2 |
| 226513 | pcDNA5/FRT_mEGFP-SNAP-tag2 | Mamalian cell expression of cytosolic mEGFP-SNAP-tag2 |
| 226514 | pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2 | Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2 |
| 226515 | pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP | Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP |
| 226516 | pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP | Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP |
| 226517 | pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL | Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum |
| 226518 | pcDNA5/FRT/TO_Vim-SNAP-tag2 | Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2 |