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Genome-wide CRISPRi-seq Identified Ferredoxin-NADP Reductase FprB as a Synergistic Target for Gallium Therapy in Pseudomonas aeruginosa.

Zhang Y, Zhang T, Xiao X, Kawalek A, Ou J, Ren A, Sun W, de Bakker V, Liu Y, Li Y, Yang L, Ye L, Jia N, Veening JW, Wang Y, Liu X
bioRxiv 2024.09.01.610675 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226434ptet-lux-Tn7T-GmThis vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.
234855Pooled sgRNA Libraries Set 1 for Pseudomonas aeruginosa PA14A CRISPRi library for inducible, genome-wide gene repression in Pseudomonas aeruginosa PA14.

Antibodies from Article