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Methods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes.

Eichler J, Huver S, Knorr CJ, Wendling C, Kobayashi T, Tomasetto C, Alpy F
Methods Mol Biol. 2025;2888:101-118. doi: 10.1007/978-1-0716-4318-1_8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226372pET28 His6-mEGFP-D4Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol
226373pET28 His6-mEGFP-D4HProduction of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Antibodies from Article