Ubiquitin modulates 26S proteasome conformational dynamics and promotes substrate degradation.
Jonsson E, Htet ZM, Bard JAM, Dong KC, Martin A
Sci Adv. 2022 Dec 23;8(51):eadd9520. doi: 10.1126/sciadv.add9520. Epub 2022 Dec 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226348 | pAM315 pACYC-His6-Rpn10[ΔUIM] | Expression of yeast Rpn10 with the UIM deleted |
| 226349 | pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y | Expression of Titin V15P substrate with GS tail |
| 226350 | pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15 | Expression of proteasome substrate with tail for molecular ruler |
| 226351 | pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10 | Expression of Titin (V15P) substrate for 26S proteasome |
| 226352 | pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5 | Expression of Titin (V15P) substrate for 26S proteasome |
| 226353 | pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1 | Expression of titin (V15P) substrate for 26S proteasome |