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Improved Fluorescent Proteins for Dual-Colour Post-Embedding CLEM.

Peng D, Li N, He W, Drasbek KR, Xu T, Zhang M, Xu P
Cells. 2022 Mar 23;11(7):1077. doi: 10.3390/cells11071077. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
226335Mito-mEosEM-EPhotoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression
226336pRSET A-mEosEM-EPhotoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression
226337mEosEM-E_N1Photoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expression
226338mEosEM-E_Lamin APhotoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

Antibodies from Article