An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in Mammalian Cells.
Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, Italia JS, Chatterjee A
Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 10.1002/anie.202219269. Epub 2023 Apr 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228780 | pAcBac1-EcTrp-TGA | aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon |
| 228783 | pAcBac1-Pyl-TAA | Pyl aaRS/tRNA system for mammalian TAA suppression |
| 228784 | pAcBac1-Pyl-TAG | Pyl aaRS/tRNA system for mammalian TAG suppression |
| 228786 | pAcBac3-EcTrp-TGA-EGFP** | EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression |
| 228787 | pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA | EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression |
| 228788 | pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA | EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression |