Biosynthesis of Macrocyclic Peptides with C-Terminal beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes.
Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA, van der Donk WA
ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 10.1021/acscentsci.4c00088. eCollection 2024 May 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226241 | pRSF_ApyAD | Production of ApyA peptide modified by the B12-rSAM enzyme ApyD |
| 226242 | pRSF_ApyAO | Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO |
| 226245 | pSCrhaB2_ApyOAHID | Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI |
| 226246 | pSCrhaB2_ApyOAHIDS | Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS |
| 226247 | pSCrhaB2_ApyD_ RBS_ApyAOHIS | Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS |
| 226248 | pRSF_ApyADO | Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO |
| 226249 | pRSF_ApyA | Production of unmodified ApyA peptide. |