Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq.
Simon JJ, Fowler DM, Maly DJ
Nat Methods. 2024 Nov;21(11):2094-2106. doi: 10.1038/s41592-024-02456-7. Epub 2024 Oct 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226162 | RNA-Protein Landing Pad | Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression |
| 226165 | (attB) tdMCP-tagged BRaf | Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter. |
| 226167 | 3xFlag-tdMCP-Erk2 (pErk reporter) | Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays. |
| 226168 | TurboID-CRaf | Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf. |
| 226169 | TurboID-MEK1 | Used to transiently express a TurboID fusion of the protein kinase MEK1. |
| 226170 | CRaf-IRES-pErk reporter | Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays. |
| 226291 | 2xMyc-tdMCP-SNAP (tdMCP standard) | Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays. |