Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii.
Strucko T, Gadar-Lopez AE, Frohling FB, Frost ET, Iversen EF, Olsson H, Jarczynska ZD, Mortensen UH
FEMS Yeast Res. 2024 Aug 23:foae026. doi: 10.1093/femsyr/foae026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226180 | pDIV151 | Expresses Cas9 in a backbone compatible with K. phaffii. |
| 226181 | pDIV270 | Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii. |
| 226182 | pDIV643 | Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii. |
| 226183 | pDIV272 | Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette. |