Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting.
Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H, Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk A, Hsu PD, Konermann S
Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 10.1016/j.cels.2023.11.006. Epub 2023 Dec 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226008 | PB_EF1a-DjCas13d-msfGFP-Blast | DjCas13d protein for RNA targeting in piggyBac backbone |
| 226009 | pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI | DjCas13d guide RNA cloning backbone (lenti) |
| 226011 | pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI | CasRx guide RNA cloning backbone (lenti) |