LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs.
Zhang J, Ataei L, Mittal K, Wu L, Caldwell L, Huynh L, Sarajideen S, Tse K, Simon MM, Mazid MA, Cook DP, Trcka D, Kwan T, Hoffman MM, Wrana JL, Esteban MA, Ramalho-Santos M
Dev Cell. 2024 Oct 14:S1534-5807(24)00574-4. doi: 10.1016/j.devcel.2024.09.024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225999 | pT2-hUbC-dCas9-KRAB-T2a-Puro | To establish stable dCas9-KRAB knock-in line |
| 226000 | pGL3-Ctr1-gRNA-EGFP | Negative control for CRISPRi-KD |
| 226001 | pGL3-Ctr2-gRNA-EGFP | Negative control for CRISPRi-KD |
| 226002 | pGL3-hL1-gRNA1-EGFP | CRISPRi-KD of human LINE1 |
| 226003 | pGL3-hL1-gRNA2-EGFP | CRISPRi-KD of human LINE1 |
| 226004 | pGL3-NCL-gRNA1-EGFP | CRISPRi-KD of human Nucleolin |
| 226005 | pGL3-NCL-gRNA2-EGFP | CRISPRi-KD of human Nucleolin |
| 226006 | pU6-hL1-5'_3.3kb plasmid | Plasmid used to generate DNA-FISH probe targeting at human L1HS |