Imran Siddiqi Lab: Siddiqi August 2024 Plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225983 | pAG1guide_RUBY_EggCas9 | To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants |
| 225984 | pCENTR_aco_mScarlet3 | Arabidopsis codon-optimized mScarlet3 in an entry clone |
| 225985 | pCENTR_DMC1p_amiRNA | To drive any artificial microRNA under the regulatory control of AtDMC1 promoter and it will make a gateway entry clone |
| 225986 | pENTR_AtU6p_BsaI_ccdb_gRNA | To make Gateway entry clone of CRISPR guide RNA under AtU6p |
| 225987 | pENTR_AtU6p_BsaI_gRNA | To make Gateway entry clone of CRISPR guide RNA under AtU6p |
| 225988 | pGENT R2 GW R1 RUBY | To express RUBY transgene under the control of desired promoter (Note: R2 Gateway R1) |
| 225990 | pGW_RUBY_EggCas9 | To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY |