Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.
Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR
bioRxiv [Preprint]. 2024 Jun 26:2024.06.25.600517. doi: 10.1101/2024.06.25.600517.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225959 | pCF140_Gag-SpyCas9-NLS | CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production. |
| 225960 | pCF142_U6-sgRNA | U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs. |
| 225961 | pCF153_Gag-Pol | CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production. |
| 225962 | pCF159_BRL | CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production. |
| 225963 | pCF160_VSV-G | CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production. |