Stress response silencing by an E3 ligase mutated in neurodegeneration.

Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rape M
Nature. 2024 Feb;626(8000):874-880. doi: 10.1038/s41586-023-06985-7. Epub 2024 Jan 31. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
226098	pcs2-GFP-IRES-mCherry	Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.
226099	pcs2-HRI-GFP-IRES-mCherry	HRI stability reporter construct for transient expression in mammalian cells
226100	pcs2-N-HRI(1-138)-GFP-IRES-mCherry	HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells
226101	pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry	HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells
226102	pcs2-cDELE1(142-515)-GFP-IRES-mCherry	cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells
226103	pcs2-MTS-cox8A-GFP-IRES-mCherry	Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells
226104	pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry	Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
226105	pcs2-ATF4uORF1-2-GFP-IRES-mCherry	ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)