Stress response silencing by an E3 ligase mutated in neurodegeneration.
Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rape M
Nature. 2024 Feb;626(8000):874-880. doi: 10.1038/s41586-023-06985-7. Epub 2024 Jan 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226098 | pcs2-GFP-IRES-mCherry | Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression. |
| 226099 | pcs2-HRI-GFP-IRES-mCherry | HRI stability reporter construct for transient expression in mammalian cells |
| 226100 | pcs2-N-HRI(1-138)-GFP-IRES-mCherry | HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells |
| 226101 | pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry | HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells |
| 226102 | pcs2-cDELE1(142-515)-GFP-IRES-mCherry | cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells |
| 226103 | pcs2-MTS-cox8A-GFP-IRES-mCherry | Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells |
| 226104 | pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry | Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells |
| 226105 | pcs2-ATF4uORF1-2-GFP-IRES-mCherry | ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR) |
| 226107 | pcs2-cDELE1(142-515)-sumo | cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays |