Barcoding Notch signaling in the developing brain.
Siniscalco AM, Perera RP, Greenslade JE, Veeravenkatasubramanian H, Masters A, Doll HM, Raj B
Development. 2024 Nov 22:dev.203102. doi: 10.1242/dev.203102.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226829 | p140Tol2-Olactb:SABER | Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2. |
| 226834 | p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA | Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish |
| 227773 | p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA | Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish |
| 227774 | p155ME-loxP-dsR-loxP-Cas9-t2A-GFP | Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning |
| 227775 | p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2 | Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2. |
| 227778 | p103DestTol2pA2-4xU6sgRNA | Destination vector with U6 driven 4 sgRNAs |