Increased cytoplasmic expression of PETase enzymes in E. coli.
Carter LM, MacFarlane CE, Karlock SP, Sen T, Kaar JL, Berberich JA, Boock JT
Microb Cell Fact. 2024 Nov 25;23(1):319. doi: 10.1186/s12934-024-02585-w.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
225759 | pET24a isPETase | Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag |
225760 | pET21b FAST-PETase | Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag |