A flexible reporter system for direct observation and isolation of cancer stem cells.
Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, Ravin R, Wakefield LM
Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225659 | 8138-E02 | Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter) |
| 225660 | 8522-M01-663 | Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration) |
| 225661 | 8522-M31-663 | Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration) |
| 225662 | 10279-M28-663 | Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding |
| 225663 | 10279-M30-663 | Lentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholding |