Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation.
Chauhan V, Baptista ISC, Arsh AM, Jagadeesan R, Dash S, Ribeiro AS
Biochemistry. 2024 Jul 12. doi: 10.1021/acs.biochem.4c00012.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225642 | ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+ | araBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225643 | PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+ | Lac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225644 | PtetA with pBELO_L6-PT3-T4_CAMR_TAN+ | tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225645 | PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+ | tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225646 | PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+ | tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225647 | PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+ | LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225648 | PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+ | LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |
| 225649 | PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+ | tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance |