Synthetically-programmed antioxidant delivery by a domesticated skin commensal

Guillermo Nevot, Javier Santos-Moreno, Nil Campamà-Sanz, Lorena Toloza, Cristóbal Parra-Cid Patrick A.M. Jansen, Içvara Barbier, Rodrigo Ledesma-Amaro, Ellen van H. den Bogaard and Marc Güell
Cell Systems, 2025 Article

Plasmids from Article

ID	Plasmid	Purpose
225602	pEND-320_Cacnes_ori	Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
225603	pEND-321_Cacnes_ori	Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
225604	pEND-349_Cacnes_ori	Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
225605	pEND-350_Cacnes_ori	Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
225606	pEND-400_Cacnes_CAMP2	Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette
225607	pEND-418_Cacnes_LysA	Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
225608	pEND-443_Cacnes_HisB	Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
225609	pEND-446_Cacnes_ProA	Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
225610	pEND-184_Cacnes_mCherry	Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
225611	pEND-224_Cacnes_sfGFP	Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
225612	pEND-225_Cacnes_mKO2	Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2
225613	pEND-226_Cacnes_mCitrine	Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine
225614	pEND-227_Cacnes_mCerulean	Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.
225615	pEND-229_Cacnes_EforCP	Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
225616	pEND-164_Cacnes_pFAST	Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
225617	pEND-351_Cacnes_dCas9_CRISPRi	Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
225618	pEND-303_Cacnes_tetR	C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
225619	pEND-304_Cacnes_tetR	C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry
225620	pEND-375_Cacnes_phlF	C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
225621	pEND-192_Cacnes_HS	C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).
225622	pEND-193_Cacnes_HS	C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)