PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato.
Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou J, Zhong Z, An X, Qi Y, Zhang Y
Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225593 | pGEL1022 | The backbone vector for rice genome editing is based on HkCas12a |
| 225594 | pGEL1023 | The backbone vector for rice genome editing is based on PrCas12a |
| 225595 | pGEL1024 | The Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12a |
| 225596 | pGEL1025 | The STU backbone vector for rice genome editing is based on Mb3Cas12a |
| 225597 | pGEL1026 | The STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R) |
| 225598 | pGEL1027 | The STU backbone vector for rice genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R) |
| 225599 | pGEL1028 | The STU backbone vector for maize genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R) |
| 225600 | pGEL1029 | The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R) |