Aggregate-selective removal of pathological tau by clustering-activated degraders.
Benn J, Cheng S, Keeling S, Smith AE, Vaysburd MJ, Boken D, Miller LVC, Katsinelos T, Franco C, Dupre E, Danis C, Landrieu I, Buee L, Klenerman D, James LC, McEwan WA
Science. 2024 Aug 30;385(6712):1009-1016. doi: 10.1126/science.adp5186. Epub 2024 Aug 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225584 | pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry | Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225585 | pcDNA4TO T21R-T2A-mCherry | Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225586 | pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry | Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225587 | pcDNA3.1 T21R-vhhGFP4-T2A-mCherry | Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225588 | pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry | Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225589 | pcDNA3.1 T21R-Nb139-T2A-mCherry | Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225590 | pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry | AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |
| 225591 | pAAV-hSyn T21R(I18R+M72E)-F8.2(S54L+T127A)-T2A-mCherry | AAV transgene plasmid with hSyn promoter for expression of catalytically dead TRIM21 RING(I18R+M72E)-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter |