An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries.
Petersen BM, Kirby MB, Chrispens KM, Irvin OM, Strawn IK, Haas CM, Walker AM, Baumer ZT, Ulmer SA, Ayala E, Rhodes ER, Guthmiller JJ, Steiner PJ, Whitehead TA
Nat Commun. 2024 May 10;15(1):3974. doi: 10.1038/s41467-024-48072-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217827 | pBDP | Stuffer plasmid with golden gate compatible bidirectional promoter |
| 217828 | pMMP_kappa | Mini-mutagenesis plasmid for kappa light chain YSD Fabs |
| 217829 | pMMP_lambda | Mini-mutagenesis plasmid for lambda light chain YSD Fabs |
| 217830 | pYSD_kappa_mRFP | Yeast surface display Fab vector with kappa light chain and mRFP |
| 217831 | pYSD_lambda_mRFP | Yeast surface display Fab vector with lambda light chain and mRFP |
| 217832 | p4A8_S7T_BC | 4A8 variant in barcoded, yeast surface display plasmid |
| 217833 | p4A8_M59I_T94M_BC | 4A8 variant in barcoded, yeast surface display plasmid |