Subunits of an E3 Ligase Complex as Degrons for Efficient Degradation of Cytosolic, Nuclear, and Membrane Proteins.
Verbic A, Lebar T, Praznik A, Jerala R
ACS Synth Biol. 2024 Mar 15;13(3):792-803. doi: 10.1021/acssynbio.3c00588. Epub 2024 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228530 | pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA | Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage. |
| 228531 | pcDNA3.1-F-box:gs10:FRB:HA | F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker. |
| 228532 | pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA | A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker. |
| 228533 | pcDNA3.1-myc:FRB:gs10:Cul1(dC501) | A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker. |
| 228534 | pcDNA3.1-myc:Rbx1:gs10:FRB:HA | Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker. |
| 228535 | pcDNA3.1-FRB:GSlinker:SSD | FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker. |