Synthetic organizer cells guide development via spatial and biochemical instructions.

Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, Lim WA
Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
225348	aTY50_pPGK1_Pcad_IRES_PuroR_WPRE	Lentiviral vector for Pcad expression
225349	aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE	Lentiviral vector for Wnt3a and BFP expression
225350	aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA	piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression
225351	aTY171_pPGK1_iRFP_WPRE	Lentiviral vector for iRFP expression
225352	aTY173_pPGK1_mTagBFP2_WPRE	Lentiviral vector for BFP expression
225353	aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE	Lentiviral vector for DKK1 and BFP expression
225355	PKMC356_pTRE-ActivinA_IRES_BFP_WPRE	Lentiviral vector for ActivinA and BFP expression