Addgene: Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary Polyphenols.

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Emily Balskus
Dong et al

Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary Polyphenols.

Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 10.1021/jacs.4c09892. Epub 2025 Feb 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
233880	pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)	Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213
233879	pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)	Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213
233877	pXD70EN25-Pct5-(–)-Eddh(Gp3C)	Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter
233876	pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)	Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213
233875	pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)	Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
233874	pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)	Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
233873	pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)	Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
233871	pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)	Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
233870	pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)	Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
233869	pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)	Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Antibodies from Article