Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform.
Zhong C, Arai S, Okada Y
Cell Rep Methods. 2024 Nov 18;4(11):100902. doi: 10.1016/j.crmeth.2024.100902.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225247 | pRSETA-qmTQ2-ATP | Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging |
| 225248 | pcDNA3.1(-)-qmTQ2-ATP | mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging |
| 225249 | pRSETA-qmTQ2-cAMP | Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP |
| 225250 | pcDNA3.1(-)-qmTQ2-cAMP | mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP |
| 225251 | pRSETA-qmTQ2-citrate | Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate |
| 225252 | pRSETA-qmTQ2-glucose | Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose |