Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing
Chey YCJ, Gierus L, Lushington C, Arudkumar JC, Geiger A, Staker LG, Robertson LJ, Pfitzner C, Kennedy JG, Lee RHB, Godahewa GI, Thomas PQ, Adikusuma F
bioRxiv 2024.07.19.604224
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178799 | PX459v3 | SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3). |
| 178800 | PX459v3-eSpCas9(1.1) | High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3). |
| 178801 | PX459v3-SpCas9-HF1 | High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3). |
| 178802 | SaCas9v2-Puro | SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. |
| 178813 | SaCas9v3-Puro | SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3). |