Phollow reveals in situ phage transmission dynamics in the zebrafish gut microbiome at single-virion resolution.
Ortiz de Ora L, Wiles ET, Zund M, Banuelos MS, Haro-Ramirez N, Suder DS, Ujagar N, Ayala-Angulo J, Trinh C, Knitter C, Gonen S, Nicholas DA, Wiles TJ
Nat Microbiol. 2025 Apr 18. doi: 10.1038/s41564-025-01981-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225185 | pTn7xTS_SpyCatcher:mNG | Used for inserting SpyCatcher fused to mNeonGreen at the bacterial Tn7 site |
| 225186 | pTn7xTS_SpyCatcher:AausFP1 | Used for inserting SpyCatcher fused to AausFP1 at the bacterial Tn7 site |
| 225187 | pTn7xTS_SpyCatcher:mCitrine | Used for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 site |
| 225188 | pTn7xTS_SpyCatcher:mKate2 | Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site |
| 225189 | pTn7xTS_SpyCatcher:mTFP1 | Used for inserting SpyCatcher fused to mTFP1 at the bacterial Tn7 site |
| 225190 | pTn7xTS_SpyCatcher003:mNG | Used for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 site |
| 225191 | pTn7xTS_SnoopCatcher:mNG | Used for inserting SnoopCatcher fused to mNeonGreen at the bacterial Tn7 site |
| 225192 | pTn7xTS_PrecA:mNeonGreen | Used for inserting PrecA upstream of mNeonGreen at the bacterial Tn7 site for use as a RecA reporter |
| 225193 | pAX1_HS_gpN:SpyTag | Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein |
| 225194 | pAX1_HS_gpN:SpyTag003 | Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein |
| 225195 | pAX1_HS_gpN:SnoopTag | Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein |
| 225196 | pAX1_Z11 gpN:SpyTag | Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein |
| 225197 | pAX1_HS∆P2 | Used to create a markerless deletion of the E. coli HS P2 phage |
| 225198 | pAX1_HS∆tum | Used to create a markerless deletion of the E. coli HS tum gene |
| 225199 | pAX1_HS_lexA(Ind-) | Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS |