Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae.
Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL
J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 10.1016/j.jbiosc.2024.08.006.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225152 | pHBJT001 | High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225153 | pHBJT002 | High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225154 | pHBJT003 | Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225155 | pHBJT004 | Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225156 | pHBJT005 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225157 | pHBJT006 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225158 | pHBJT007 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225159 | pHBJT008 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225160 | pHBJT009 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225161 | pHBJT010 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225162 | pHBJT011 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225163 | pHBJT012 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225164 | pHBJT013 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter. |
| 225165 | pHBJT014 | Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter. |
| 225166 | pHBJT015 | Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (ChlR). |
| 225167 | pHBJT016 | Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (ChlR). |
| 225168 | pHBJT017 | Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (AmpR). |
| 225169 | pHBJT018 | Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (AmpR). |
| 225170 | pHBJT019 | Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (TetR). |
| 225171 | pHBJT020 | Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR). |
| 225172 | pHBJT021 | Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (GmR). |
| 225173 | pHBJT022 | Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (GmR). |
| 225174 | pHBJT023 | Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR). |
| 225175 | pHBJT024 | Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (SmR). |
| 225176 | pHBJT030 | Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions. |
| 225177 | pHBJT031 | Low copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions. |
| 225178 | pHBJT032 | Low copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions. |
| 225179 | pHBJT033 | Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions. |
| 225180 | pHBJT034 | Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions. |