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RpH-ILV: Probe for lysosomal pH and acute LLOMe-induced membrane permeabilization in cell lines and Drosophila.

Cheetham-Wilkinson IJ, Sivalingam B, Flitton C, Flottmann F, Vehling L, Drechsler M, Stojchevska M, Raimondi A, Paululat A, Frohlich F, Swan LE, Stagi M
Sci Adv. 2025 Jan 3;11(1):eadr7325. doi: 10.1126/sciadv.adr7325. Epub 2025 Jan 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225135RpH-ILV-3xALFARpH-ILV probe strongly colocalises with lysosomal markers.
225136R2pH-LAMPx3ALFAR2pH-LAMP1-labelled lysosomes with 3xLFA
225137RpH-ILV-FRBRpH-ILV probe strongly colocalises with lysosomal markers with FRB
225138R2pH-LAMP-FRBR2pH-LAMP1-labelled lysosomes with FRB

Antibodies from Article