Establishing a straightforward I-SceI-mediated recombination one-plasmid system for efficient genome editing in P. putida KT2440.
Meng H, Kobbing S, Blank LM
Microb Biotechnol. 2024 Jul;17(7):e14531. doi: 10.1111/1751-7915.14531.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225132 | pEMG-RIS | For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km. |
| 225133 | pEMG-AIS | For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km. |
| 225134 | pEMG-GRIS | For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm. |