Diego Orzaez Lab: CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation
Elena Garcia-Perez
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225430 | pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642) | OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter |
| 225431 | pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641) | OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter |
| 225432 | pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598) | Transcriptional unit of BeYDV Rep/RepA (C1:C2) |
| 225433 | pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625) | Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper. |
| 225434 | pDGB3_alpha1_35S-eGFP-T35S (GB4279) | Transcriptional unit for constitutive expression of the enhanced GFP. |
| 225435 | pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312) | BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2. |
| 225436 | pDGB3_alpha2_BeYDV geminino 1.0 (GB4480) | BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription. |
| 225437 | pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643) | Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA. |
| 225438 | pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646) | Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR. |
| 225439 | pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684) | Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette. |
| 225440 | pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697) | Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette. |
| 225441 | pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954) | Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette. |
| 225442 | pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177) | BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration. |
| 225443 | pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178) | Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1. |