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Macrocyclization and Backbone Rearrangement During RiPP Biosynthesis by a SAM-Dependent Domain-of-Unknown-Function 692.

Ayikpoe RS, Zhu L, Chen JY, Ting CP, van der Donk WA
ACS Cent Sci. 2023 Apr 24;9(5):1008-1018. doi: 10.1021/acscentsci.3c00160. eCollection 2023 May 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225080pACYCDuet-1:ChrA-C63SExpresses precursor peptide ChrA-C63S in E.coli
225083pCDFDuet-1:ChrH-ChrIExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli
225084pET28a:ChrHExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli
225085pET28a:ChrIExpresses anthrone-oxygenase like enzyme ChrI in E.coli

Antibodies from Article