A scalable platform to discover antimicrobials of ribosomal origin.
Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225065 | pET28:LanI-101 | Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli |
| 225066 | pET28:LanII | Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli |
| 225067 | pET28:LanII-23 | Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli |
| 225068 | pET28:LanII-2A | Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli |
| 225069 | pET28:LanII-2B | Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli |
| 225070 | pET28:LanII-2C | Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli |
| 225071 | pET28:LanII-2D | Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli |
| 225072 | pET28:LanII-2E | Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli |
| 225073 | pET28:LanII-56 | Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli |
| 225074 | pET28:LanII-57 | Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli |
| 225076 | pET28:LanIII-6 | Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli |
| 225077 | pET28:LanIII-43 | Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli |
| 225078 | pET28:LanIV-Chondromyces | Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli |