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Addgene

A scalable platform to discover antimicrobials of ribosomal origin.

Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225065pET28:LanI-101Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli
225066pET28:LanIIExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli
225067pET28:LanII-23Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli
225068pET28:LanII-2AExpresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli
225069pET28:LanII-2BExpresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli
225070pET28:LanII-2CExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli
225071pET28:LanII-2DExpresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli
225072pET28:LanII-2EExpresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli
225073pET28:LanII-56Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli
225074pET28:LanII-57Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli
225076pET28:LanIII-6Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli
225077pET28:LanIII-43Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli
225078pET28:LanIV-ChondromycesExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli
226250pET28:LanIV-StreptomycesExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Antibodies from Article