A split-luciferase lysate-based approach to identify small-molecule modulators of phosphatase subunit interactions.
Claes Z, Bollen M
Cell Chem Biol. 2023 Dec 21;30(12):1666-1679.e6. doi: 10.1016/j.chembiol.2023.07.018. Epub 2023 Aug 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224893 | pET16b-MYPT1(1-314) | Bacterial expression of MYPT1(1-314) |
| 224894 | pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag | Mammalian expression of LgBiT-MYPT1(1--314) |
| 224895 | pEGFP-C1-Flag-SmBiT-PP1b | Mammalian expression of Flag-SmBiT-PP1b |