Paul Herman Lab: Herman lab plasmids 2024

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
224875	pRS416 GAL1pro-YFP	Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)
224876	pRS416 CUP1pro-Htt103QdeltaP161-170-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.
224878	pRS416 CUP1pro-Htt103QdeltaP135-170-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.
224880	pRS416 CUP1pro-Htt103QdeltaP141-170-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.
224882	pRS416 CUP1pro-Htt103QdeltaP125-134-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.
224883	pRS416 CUP1pro-Htt103QdeltaP125-160-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.
224885	pRS416 CUP1pro-M-PRR-Htt97QP4-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.
224886	pRS416 CUP1pro-Htt103QdeltaP141-160-YFP	Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.
224887	pRS415 GPDpro-RNQ1-CFP	Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).