Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing.
Kiyokawa K, Sakuma T, Moriguchi K, Sugiyama M, Akao T, Yamamoto T, Suzuki K
Appl Microbiol Biotechnol. 2024 Jul 12;108(1):416. doi: 10.1007/s00253-024-13242-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224867 | pBSsgRNA | Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene |
| 224868 | pYAMTr2GCsgScLEU2 | Disruption of S. cerevisiae type LEU2 gene |
| 224869 | pYAMTrGCsgScLEU2 | Disruption of S. cerevisiae type LEU2 genes |
| 224870 | pYAMTr2GCsgSeLEU2 | Disruption of S. eubayanus type LEU2 genes |
| 224871 | pYAMTr2GCsgSc-SeLEU2 | Disruption of LEU2 genes in the Saccharomyces sense strict group species |