Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting.
Wan Y, Helenek C, Coraci D, Balazsi G
ACS Synth Biol. 2024 Oct 18;13(10):3212-3230. doi: 10.1021/acssynbio.4c00271. Epub 2024 Oct 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224781 | LPUtopia-8 | Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells |
| 224782 | LPUtopia-7_CoV-2 | Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay |
| 224783 | pUC-hU6-2O-crBFP_EF1a-BFP | BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter |
| 224784 | pUC-hU6-2O-crEGFP_EF1a-BFP | EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter |
| 224785 | pUC-hU6-crBFP_EF1a-BFP | BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter |
| 224786 | pUC-hU6-CrEGFP_EF1a-BFP | EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter |
| 224787 | pUC-hU6-crBACH1-array_EF1a-BFP | BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter |
| 224788 | pUC-hU6-crCoV-Mutiplex_EF1a-BFP | SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter |
| 224789 | pUt-mNF-Cas13d | LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection. |
| 224790 | pUt-MONARCH 1.0_crEGFP | LPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selection |
| 224793 | pUt-MONARCH 2.0_crEGFP | LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP. |
| 224860 | pUC-hU6-crScaffold_SV40-BFP | crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter |
| 224861 | pUC-hU6-2O-crScaffold_SV40-BFP | crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter |
| 224862 | pUC-hU6-2O-crScaffold_EF1a-BFP | crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter |
| 227709 | pUT_mNF-Cas13d-Triplex | LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system. |