Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates.
Uckelmann M, Levina V, Taveneau C, Han Ng X, Pandey V, Martinez J, Mendiratta S, Houx J, Boudes M, Venugopal H, Trepout S, Zhang Q, Flanigan S, Li M, Sierecki E, Gambin Y, Das PP, Bell O, de Marco A, Davidovich C
bioRxiv [Preprint]. 2024 Feb 12:2023.05.08.539931. doi: 10.1101/2023.05.08.539931.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224706 | pET3a.H2B-K120C | Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers |
| 224707 | pFB1.HMBP.PrS.CBX8 | Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 224708 | pFB1.HMBP.PrS.CBX8ΔChromo | Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag |
| 224709 | pFB1.HMBP.PrS.CBX8-KR21A | Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 224710 | pFBOH-MHL.mEGFP-CBX8 | Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag. |
| 224711 | pFBOH-MHL.BMI1 | Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells. |
| 224712 | pFBOH-MHL.RING1B | Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells. |