SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, Voeltz GK
Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 10.1016/j.devcel.2024.06.005.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226562 | Lyric-mNeonGreen | Encodes Lyric protein labeled with mNeonGreen |
| 226563 | Lyric-mCherry | Encodes Lyric protein labeled with mCherry |
| 226564 | mNeonGreen-Lrrc59 | Encodes Lrrc59 protein labeled with mNeonGreen |
| 226565 | mCherry-Lrrc59 | Encodes Lrrc59 protein laebeled with mCherry |
| 226566 | SigmaR1-mNeonGreen | Encodes SigmaR1 protein labeled with mNeonGreen |
| 226567 | siRNA-resistant SigmaR1-mCherry | Encodes siRNA resistant SigmaR1 protein labeled with mCherry |
| 226568 | mNeonGreen-KDEL | Encodes mNeonGreen with KDEL ER lumenal target sequence |
| 226569 | siRNA-resistant pLV-SigmaR1-mNeonGreen | Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector) |
| 226571 | siRNA-resistant SigmaR1-mNeonGreen F191A | Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen |
| 226572 | siRNA-resistant SigmaR1-mNeonGreen R119A | Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen |
| 226573 | siRNA-resistant SigmaR1-mNeonGreen D188A | Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen |
| 226574 | siRNA-resistant SigmaR1-mNeonGreen R119A F191A | Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen |
| 226575 | ss-SigmaR1DTM-mNeonGreen | Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen |
| 226576 | siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A | Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen |
| 226577 | siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A) | Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen |
| 226578 | siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A) | Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen |