SigmaR1 shapes rough endoplasmic reticulum membrane sheets.

Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, Voeltz GK
Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 10.1016/j.devcel.2024.06.005. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
226578	siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)	Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
226577	siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)	Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
226576	siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A	Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen
226575	ss-SigmaR1DTM-mNeonGreen	Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen
226574	siRNA-resistant SigmaR1-mNeonGreen R119A F191A	Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
226573	siRNA-resistant SigmaR1-mNeonGreen D188A	Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen
226572	siRNA-resistant SigmaR1-mNeonGreen R119A	Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen
226571	siRNA-resistant SigmaR1-mNeonGreen F191A	Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen
226569	siRNA-resistant pLV-SigmaR1-mNeonGreen	Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)
226568	mNeonGreen-KDEL	Encodes mNeonGreen with KDEL ER lumenal target sequence