Development of covalent chemogenetic K(2P) channel activators.
Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr
Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 10.1016/j.chembiol.2024.06.006.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224744 | pGEMHE:mTREK-1(S131C) | In vitro transcription for Oocyte expression of mTREK-1(S131C) |
| 224745 | pGEMHE:mTREK-1(S131A) | In vitro transcription for Oocyte expression of mTREK-1(S131A) |
| 224746 | pGEMHE:mTREK-1(A286F) | In vitro transcription for Oocyte expression of mTREK-1(A286F) |
| 224747 | pGEMHE:mTREK-1(G171F) | In vitro transcription for Oocyte expression of mTREK-1(G171F) |
| 224748 | pGEMHE:mTREK-1(S131C/A286F) | In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F) |
| 224749 | pGEMHE:mTREK-1(S131C/G171F) | In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F) |
| 224750 | pGEMHE:mTREK-1(S131C/K271Q) | In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q) |
| 224751 | pGEMHE:mTREK-1 (C159A/C219A) | In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A) |
| 224752 | pGEMHE:mTREK-1 (S131C/C159A/C219A) | In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A) |
| 224753 | pGEMHE:mTREK-1 (S131A/C159A/C219A) | In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A) |
| 224754 | pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A) | In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A) |
| 224755 | pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A) | In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A) |
| 224756 | pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A) | In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A) |
| 224758 | pGEMHE:mTREK-1(tandem S131C-wt) | In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt) |
| 224759 | pGEMHE:mTREK-1(tandem wt-S131C) | In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C) |
| 224760 | pGEMHE:mTREK-1(tandem S131C-S131C) | In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C) |
| 224761 | pGEMHE:hTRAAK | In vitro transcription for Oocyte expression of hTRAAK |
| 224762 | pGEMHE:hTRAAK(S118C) | In vitro transcription for Oocyte expression of hTRAAK(S118C) |
| 224765 | pGEMHE:mTRAAK | In vitro transcription for Oocyte expression of mTRAAK |
| 224766 | pGEMHE:mTRAAK(S93C) | In vitro transcription for Oocyte expression of mTRAAK(S93C) |
| 224767 | pGEMHE:mTRAAK(Q233K) | In vitro transcription for Oocyte expression of mTRAAK(Q233K) |
| 224768 | pGEMHE:mTRAAK(S93C/Q233K) | In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K) |
| 224769 | pcDNA3.1 TREK-2 | In vitro transcription for Oocyte expression of 1 TREK-2 |
| 224770 | pcDNA3.1 TREK-2(S156C) | In vitro transcription for Oocyte expression of 1 TREK-2(S156C) |
| 224773 | pIRES2-eGFP:HA-mTREK-1 | Mammalian expression vector for generating mouse TREK-1 |
| 224774 | pIRES2-eGFP:HA-mTREK-1(S131C) | Mammalian expression vector for generating mouse TREK-1 |
| 224775 | pIRES2-eGFP:HA-mTREK-1(S131C/A286F) | Mammalian expression vector for generating mouse TREK-1 |
| 224776 | pIRES2-eGFP:HA-mTREK-1(tandem wt-wt) | Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker |
| 224777 | pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt) | Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker |
| 224778 | pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C) | Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker |
| 224779 | pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C) | Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker |
| 224780 | pPICZ:K2P2.1(mTREK-1)Cryst S112C | Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter |