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Development of covalent chemogenetic K(2P) channel activators.

Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr
Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 10.1016/j.chembiol.2024.06.006. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
224743pGEMHE:mTREK-1In vitro transcription for Oocyte expression of mTREK-1
224744pGEMHE:mTREK-1(S131C)In vitro transcription for Oocyte expression of mTREK-1(S131C)
224745pGEMHE:mTREK-1(S131A)In vitro transcription for Oocyte expression of mTREK-1(S131A)
224746pGEMHE:mTREK-1(A286F)In vitro transcription for Oocyte expression of mTREK-1(A286F)
224747pGEMHE:mTREK-1(G171F)In vitro transcription for Oocyte expression of mTREK-1(G171F)
224748pGEMHE:mTREK-1(S131C/A286F)In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)
224749pGEMHE:mTREK-1(S131C/G171F)In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)
224750pGEMHE:mTREK-1(S131C/K271Q)In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)
224751pGEMHE:mTREK-1 (C159A/C219A)In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)
224752pGEMHE:mTREK-1 (S131C/C159A/C219A)In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)
224753pGEMHE:mTREK-1 (S131A/C159A/C219A)In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)
224754pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)
224755pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)
224756pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)
224758pGEMHE:mTREK-1(tandem S131C-wt)In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)
224759pGEMHE:mTREK-1(tandem wt-S131C)In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)
224760pGEMHE:mTREK-1(tandem S131C-S131C)In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)
224761pGEMHE:hTRAAKIn vitro transcription for Oocyte expression of hTRAAK
224762pGEMHE:hTRAAK(S118C)In vitro transcription for Oocyte expression of hTRAAK(S118C)
224763pGEMHE:hTRAAK(Q258K)In vitro transcription for Oocyte expression of hTRAAK(Q258K)
224764pGEMHE:hTRAAK(S118C/Q258K)In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)
224765pGEMHE:mTRAAKIn vitro transcription for Oocyte expression of mTRAAK
224766pGEMHE:mTRAAK(S93C)In vitro transcription for Oocyte expression of mTRAAK(S93C)
224767pGEMHE:mTRAAK(Q233K)In vitro transcription for Oocyte expression of mTRAAK(Q233K)
224768pGEMHE:mTRAAK(S93C/Q233K)In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)
224769pcDNA3.1 TREK-2In vitro transcription for Oocyte expression of 1 TREK-2
224770pcDNA3.1 TREK-2(S156C)In vitro transcription for Oocyte expression of 1 TREK-2(S156C)
224772pcDNA3.1:mTRESK(S116C)In vitro transcription for Oocyte expression of mTRESK(S116C)
224773pIRES2-eGFP:HA-mTREK-1Mammalian expression vector for generating mouse TREK-1
224774pIRES2-eGFP:HA-mTREK-1(S131C)Mammalian expression vector for generating mouse TREK-1
224775pIRES2-eGFP:HA-mTREK-1(S131C/A286F)Mammalian expression vector for generating mouse TREK-1
224776pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker
224777pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker
224778pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker
224779pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker
224780pPICZ:K2P2.1(mTREK-1)Cryst S112CPichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter
225490pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFPNeuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

Antibodies from Article