CRISPR-Cas13d as a molecular tool to achieve targeted gene expression knockdown in chick embryos.
Kim M, Hutchins EJ
Dev Biol. 2024 Nov 30:S0012-1606(24)00265-3. doi: 10.1016/j.ydbio.2024.11.013.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224564 | pCAG-memRFP | Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter. |
| 224565 | pCAG-Cas13d-T2A-Citrine | Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter. |
| 224566 | pCAG-Cas13d-T2A-GFP(1-10) | Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter. |
| 224567 | pCAG-memRFP-3xControlgRNA | Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter. |
| 224568 | pCAG-nucGFP11-3xControlgRNA | Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter. |
| 224569 | pCAG-memRFP-3xPax7gRNA | Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter. |
| 224570 | pCAG-nucGFP11-3xPax7gRNA | Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter. |
| 224571 | pCAG-3xGFP11-mem-3xPax7gRNA | Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter. |
| 224583 | pCAG-3xGFP11-mem-3xControlgRNA | Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter. |