Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems
Gillespie W, Zhang Y, Ruiz OE, Cerda III J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien C-B, Kwan KM, Arenkiel BR, Devine WP, Wythe JD
bioRxiv 2024.07.13.603267
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224507 | pME TAEL2.0-5xC120-minpro (JDW 1109) | Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone) |
| 224508 | pME-TAEL (JDW 1324) | Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver) |
| 224509 | pME-myc-BirA (JDW 1210) | Gateway compatible middle entry clone containing a Myc tagged BirA |
| 224510 | pME-Myc-BioID2-MCS1 (JDW 1095) | Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct |
| 224511 | pME-Myc-BioID2-MCS2 (JDW 1209) | A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS |
| 224512 | pME-3xHA-BirA-T2A-mCherry-caax (JDW 889) | A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter) |
| 224513 | pME-3x-FLAG-Ubiquitin (JDW 1098) | A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status |
| 224514 | pME-3xNLS-(G2SG2)2-Cre (JDW 1149) | A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre |
| 224515 | pME nls EGFP-Cre (JDW 1329) | A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion |
| 224516 | pME-mScarlet-I-P2A-iCre (JDW 917) | A Gateway compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized) |
| 224517 | pME-3xNLS-mTagBFP2-Cre (JDW 918) | A Gateway compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion |
| 224518 | pME-mTurquoise-P2A-iCre (JDW 920) | A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre) |
| 224520 | pME iCre-I (JDW 1203) | A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria |
| 224522 | pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232) | A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette |
| 224523 | pME-DreERT2 (JDW 1344) | A Gateway compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity |
| 224525 | pME EFS-rtTA/rtTA3 (JDW 1230) | A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator |
| 224528 | p3E-H2A_mCherry_SV40pA (JDW 967) | A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA |
| 224529 | p3E-V5-mScarlet-I_SV40pA (JDW 968) | A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA |
| 224530 | p3E-V5-mClover3-pA (JDW 1318) | A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA |
| 224531 | p3E-V5-mTagBFP2 (JDW 871) | A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2 |
| 224532 | p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256) | A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA |
| 224533 | p3E EFS-rtTA/rtTA3 (JDW 1214) | A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator |
| 224534 | p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225) | A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag |
| 224535 | p3E-IRES-mTagBFP2-pA (JDW 1361) | A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA |
| 224536 | p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360) | A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3 |
| 224537 | p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364) | A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA |
| 224538 | pDONR221-Lifeact- mScarlet-HA (JDW 1247) | A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter) |
| 224543 | pME H2B mCerulean (JDW 1150) | A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus |
| 224544 | p5E 5X C120 c-fos min pro (JDW 1241) | A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter |
| 224545 | p3E-WPRE-SV40-pA (JDW 922) | A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA |