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Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome.

Metzner E, Southard KM, Norman TM
bioRxiv [Preprint]. 2024 Jul 27:2024.07.26.605307. doi: 10.1101/2024.07.26.605307. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
224898pEM040 [Multiome Perturb-seq sgRNA expression vector]Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
224899pEM047 [T7-assisted CROPseq sgRNA expression vector]Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

Antibodies from Article